IM-01 - Advances in 3-dimensional image reconstruction

The session will cover the quantitative analysis of X-ray and electron based 3D microscopy data. Electron and X-ray 3D microscopy is widely used in material science and is an emerging field with great potential in bioimaging. With the possibility of imaging 3D structures at high spatial or temporal resolution both ex and in vivo allows structural quantification that has many applications in bioimaging. In situ 3D imaging within materials science and 3D imaging of the interface between biology and materials science often requires imaging at different length scales. The full potential of electron and X-ray 3D microscopy can only be utilized having automated analysis methods and subsequent modeling tools. This session will focus on recent developments within X-ray 3D microscopy for both laboratory and large-scale synchrotron facilities as well as applications of 3D imaging techniques by electron sources.

IM-02 - Innovative, modular and adaptable hardware: design, application and control 

Developments in electronics, manufacturing and modular components have lowered the barrier for microscopists to design, build and test their own innovative instrumentation. Advances in scripting, open control libraries (e.g. Python), have enabled users to control their instruments in more automated, accessible and flexible ways. In parallel, the drive towards inter-operable systems motivates the use of standardised ports, flanges and connectors. This session will explore innovations in low-cost, standardised, modular, and/or retrofitted and repurposed hardware, as well as novel methods of instrument control, acquisition or automation.

IM-03 - Phase-related methods

This session will cover new developments in phase-related imaging techniques across electron microscopy, x-ray imaging and allied techniques. With the proliferation of high-speed detectors, we are increasingly able to (i) make use of phase-structured illumination in the imaging process, and (ii) employ novel reconstruction algorithms to extract both the phase and the amplitude of the transmitted beam – revealing samples in increasingly complex detail. In this session, we look forwards to exploring the synergy of these two approaches. We seek submissions relating to phase retrieval methods (i.e. ptychography, (Lorentz) DPC-STEM, TIE), and submissions relating to phase shaping as employed in the imaging process (holographic and interferometric methods, vortex beams and other probe shapes).

IM-04 - New Instrumentation

Recent technological breakthroughs for both imaging and spectroscopy using electrons, ions, photons and X-rays, have provided a fertile new ground for correlative analysis of materials' structural, chemical and physical properties together with their functionalities at unprecedented spatial and spectral resolution. For this, state-of-the-art approaches to sample preparation, fabrication, and analytics that go beyond conventional methods need to be developed. This session will provide a forum to review and discuss recent scientific achievements at the forefront of new instrumentation development and the impact of these new instruments, where topical areas include: high-brightness sources, high-energy resolution monochromators, structured illumination, adaptive optics, novel aberration correctors, spectrometers, fast and collection-efficient detectors, as well as innovative focused ion-beam tools.

IM-05 - Spectroscopy and hyperspectral imaging of hard and soft matter

Hyperspectral imaging has become ubiquitous in a wide range of techniques to couple spatial and spectral information at the micro- down to atomic scale. This session aims at exploring the latest advances in spectroscopies, including hyperspectral data acquisition, processing, and analysis on hard and soft matter, by electron, ion, and x-ray microscopy. It covers the recent developments of data acquisition schemes, such as low dose, random scan, multi-signal, etc., using spectroscopies like EELS, EDX/WDX, IR, Raman, He-Ne, XAS (soft and hard x-rays). Studies with focus on hyperspectral data analysis and modelling, using e.g. inelastic channeling, atomistic calculations, machine learning algorithms, etc. are strongly encouraged, in addition to contributions involving the development of novel hyperspectroscopy methods. Related Conference Theme/s: Imaging Quantum Phenomena. The Lab in the Microscope - In Situ, In Vivo, In Operando and Multimodal Microscopy

IM-06 - Diffraction Techniques and Structural Analysis

In recent years structural analysis and nanoscale crystallography using electron diffraction and related techniques has been transformed through a series of major developments, including 3D ED/MicroED; the advent of remarkably sensitive, almost noise-free, cameras and detectors; the introduction of automated data collection and big data processing (e.g. machine learning); and the availability of dedicated software that enables robust and reliable structure determination from electron diffraction data. This has led to an explosion of interest in both the physical and life sciences in using these techniques to (i) solve crystal structures not amenable to more conventional x-ray diffraction and (ii) use the high spatial resolution of the electron microscope to reveal hitherto unseen micro- and nano-structure using 4D scanning methods, acquiring diffraction patterns at every real space probe position. This session highlights the progress made in this area using (scanning) transmission electron microscopy including 3D-ED/MicroED, 4D-STEM, SerialED, SED, SPED, PED, CBED, SCBED, HRTEM, time-resolved diffraction and ptychography, and in scanning electron microscopy (with techniques such as EBSD (2D and 3D), ECCI and TKD.

IM-07 - Dynamic studies using micro-nano labs 

Recent significant developments in electron microscopy, x-ray and scanning probe instrumentation with new in situ platforms and fast detectors enable in situ and operando experiments to be performed with high spatial-temporal resolution imaging, diffraction and spectroscopy with unprecedented detail. This provides us the opportunity to glimpse into the state and functionality of materials during synthesis, operation or degradation in a defined environment. In this symposium, we aim to highlight novel approaches to in situ microscopy enabled, e.g., by nanofabricated devices such as microchip systems and 2D material devices. Applications range from material processes studied under various stimuli including gas, temperature, liquid and mechanical, but also from the increasing use of these capabilities in life science and soft matter. We seek contributions illustrating equipment development and novel use of existing equipment both in TEM and SEM, x-ray platforms and scanning probe systems, that can provide new insight into the state of active materials including novel bio-, soft and hard materials, nanoparticles and catalysts.

IM-08 - Fast and Ultrafast Dynamics using Transmission Electron Microscopy 

This session will focus on advances in the study of fast and ultrafast chemical and materials dynamics (structural, electronic, magnetic) with transmission electron microscopy instrumentation and methods. Abstracts are encouraged in (but not limited to) the following areas:
Techniques and instrumentation for high frame rate imaging and the science they enable. Method and instrument development for increasing spatial, temporal, and energy resolutions in ultrafast transmission electron microscopes (UTEMs), both in stroboscopic and single-shot modes. Pulse compression methods and schemes, including those for generating attosecond electron packets in the stroboscopic mode and sub-nanosecond packets in the single shot mode. Laser technology/architecture and other means for generating discrete electron packets (e.g., RF cavities) that expand UTEM capabilities and the application space. UTEM-compatible cathode developments; including those based on swept beams, field emission, Schottky-type, and thermionic electron guns. Discoveries, new physical insights, and paradigm tests that have occurred because of, and have been enabled by, UTEM developments and advancements. In addition to communicating technology developments and the new scientific advances resulting from UTEM experiments, a goal of this symposium is to stimulate discussions on future directions of fast and ultrafast TEM and to foster the formation of new collaborations and new ideas within the community. Related Conference Theme/s: Live & Fast Super-resolution - Frontiers in Imaging of Ultrafast Processes. Imaging Quantum Phenomena.

IM-09 - Scanning Probe Microscopy: Imaging and Beyond

This session will focus on the last cutting-edge developments of scanning probe microscopies for the characterisation of materials, from soft biological and polymeric materials to hard inorganic materials. Beyond surface imaging, the session will highlight the abilities of SPM to characterise the surface properties of materials with a particular focus on the nanomechanical property characterisation with various AFM-based techniques and on the functional properties such as electronic, magnetic, piezoelectric properties with techniques such as EFM, KPFM, MFM, PFM, sMIM, ...

IM-10 - Machine Learning-based processing and analysis of microscopy data

Cutting-edge microscopy couples state-of-the-art instruments and machine learning-based data processing and analysis methods to enable novel discoveries from the imaged samples. Recent developments in methods, availability of open datasets, as well as advances in computing capabilities have enabled the application of machine learning for the processing of microscopy datasets. Furthermore, the development of sustainable, open-source and user-friendly software is of paramount importance to make these algorithms and workflows widely available in the scientific community and to promote reproducible research. This session will feature recent progress in machine learning-based analysis methods, processing workflows, and open-source software in microscopy.

IM-11 - Advances in SPA Cryo Electron Microscopy

This session is specifically targeted at innovations in cryo electron microscopy, supporting instrumentation and software analysis tools critical to the delivery of high-resolution cryo electron microscopy Single Particle Analysis and MicroED results.The power of single particle techniques has driven a resolution revolution in electron microscopy. However, this method is dependent on sample preparation and sample quality. Poor sample quality, lack of reproducibility and a high skill barrier to the use of supporting technologies is often limiting the potential of the cryo electron microscope. New innovations in preparing cryo-EM grids, development of new detectors, STEM imaging and automatic data screening and collection are examples of the development in the cryo-EM field.

IM-13 - Development of Sample preparation method for volume electron microscopy

Volume EM has been chosen by Nature as one of the 7 techniques to watch in 2023. In all these techniques ( tomography, serial sections TEM, array tomography, FIBSEM, SBF-SEM) allowing to visualize large volumes at ultrastructural level, sample preparation is a critical step that determines the quality and accuracy of the resulting images. The goal of sample preparation for vEM is to preserve the sample ultrastructure, provide a high contrast and produce high-quality images .The images can then be reconstructed into 3D volumes using computational methods. In this session, we will share our experiences on sample preparation, their strengths and challenges, the critical steps and the novelties. As vEM is often used in a correlative approach, compatibilities of protocols with other image modalities (light microscopy, X-ray, etc..) will be discussed.

IM-14 - Development and application of instrumentation, workflows, and data solutions for VolumeEM 

VolumeEM refers to a family of techniques that extent traditional 2D cross-section electron microscopy into the third dimension. VolumeEM encompasses techniques like serial blockface SEM, FIB-SEM, serial section SEM or TEM, grid-tape TEM, and multi-beam SEM. VolumeEM stands to revolutionize bio-imaging by supplying high-resolution volumetric biological data within the 3D context of cells, tissues, organs, and organisms. At present, however, VolumeEM also still faces challenges, e.g., related to throughput, 3D resolution, ROI targeting, system stability during long acquisitions, data consistency, and handling and analyzing massive amounts of data. This symposium brings together developments in instrumentation, workflows, data handling and analysis for VolumeEM, and their application to uncover new insights in biology and related fields.

IM-15 - Multiparameter Imaging for High Dimensional Data Acquisition

Obtaining spatially resolved microscopy data and information from cells, tissue and organisms is the key for basic and translational research, but also for diagnostics. New developments in instrumentation and sample preparation enable novel approaches for multimodal and multiparametric imaging. With these technologies, it is possible to acquire genomic, proteomic data and to place this information in a larger structural context. New sample preparation and multimodal imaging modalities are discussed towards multi-omics microscopy.Abstracts are encouraged in and around this area.

IM-16 - Correlative Microscopy Across the Scales 

Combining complementary imaging modalities can generate a highly informative, composite view of the sample and allows access to both structural and functional parameters across all relevant scales from the same sample . While the correlation of light with transmission electron microscopy (EM) is well established (generally referred to as CLEM), the frontiers of bioimaging are currently being pushed towards the integration of other modalities into correlative workflows, including both super-resolution and large-scale imaging techniques such as light sheet, X-ray or Volume EM to study intercellular relationships and put molecular information into its overall structural context.