LS-02:
Adding Dimensions to Intravital Imaging to Better Eavesdrop on Biology
Teaser:
We are seeking better solutions to the many compromises involved in the “pyramid of frustration” in which biological imagers must live. Our faster and more efficient light sheet and laser-scanning microscopes offer a better balance of spatial & temporal resolution, field of view, limited photon budget, and multiplex detection.
LS-02:
Mechanics of blastocyst
morphogenesis
Teaser:
Mammalian embryos develop over many days while relying on short-lived cellular processes to change shape.
Therefore, understanding how cells sculpt the mammalian embryo during its development requires imaging shape changes across time scales. We use microscopy, genetics and biophysics tools to understand the forces controlling the early development of mammals, including humans.
LS-03:
Dynamics of molecules at the nanoscale: from RESOLFT to STARSS
Teaser:
Fluorescence microscopy is pivotal to the investigation of the dynamics of macromolecules. We introduce new imaging methodologies based on photo-switching to address the dynamics of molecules at the nanoscale.
LS-03:
Imaging sub-cellular events at high resolution using advanced light microscopy
Teaser:
Expansion microscopy is a recently developed technique that physically magnifies biological samples, enabling super-resolution visualisation of cells using a standard microscope. Here I will present the latest optimisations in this field, aimed at preserving cell ultrastructure in an optimal manner and revealing the cellular context previously invisible under fluorescence microscopy. To demonstrate the effectiveness of expansion microscopy, I will present various applications ranging from elucidating the architecture of the centriole to exploring its potential in gene therapy treatments.
LS-03:
From DNA Nanotechnology to biomedical insight: Towards single-molecule spatial omics
Teaser:
The talk shows how DNA-barcoding and RESI enhance
fluorescence microscopy to
Ångström-scale resolution with standard equipment.
The technique achieves single-protein resolution in cells and maps CD20 in immunotherapy research, bridging super-resolution microscopy and structural biology.
LS-04:
Correlative Organelle Microscopy: Molecular, functional and ultrastructural characterization of organelles
Teaser:
TBA
Oda Helene Schiøtz
LS-04:
Targeting in cryo-ET sample preparation of large volume samples
Teaser:
TBA
LS-05:
Localization techniques in biomedical electron microscopy:
good as gold
Teaser:
You want to find out where your protein of interest is localized in the tissue or cell?
You also need information about the ultrastructure of the environment of this protein? Then you should attend this introduction into localization techniques in biomedical electron microscopy with examples from real life lab experiences!
LS-06:
Viral replication organelles revisited by cryo-electron microscopy
Teaser:
Explore the microscopic world of infected cells, where RNA viruses like coronaviruses build up their own replication fortresses. Discover how cutting-edge cryo-EM technology is unveiling macromolecular secrets of these viral replication organelles, opening venues to disrupt viral replication and disease.
LS-07:
A multimodal in situ characterization of alveoli architecture and function in healthy, diseased and toxicant-induced damaged states
Teaser:
TBA
LS-07:
Volume Correlative Light and Electron Microscopy (vCLEM): greater than the sum of its parts
Teaser:
TBA
LS-08:
Integrated structural cell biology of pathogen-host interactions
Teaser:
Intracellular pathogens hijack and remodel host cell machineries to promote their replication. Using an integrative structural cell biology approach centred around electron cryo tomography we here reveal molecular insights into some of the respective mechanisms employed by herpesviruses and the malaria parasite Plasmodium falciparum to modulate cellular membranes and microtubules, respectively.
LS-09:
Structural insights into protein biogenesis at organelles
Teaser:
Many freshly synthesized proteins must be translocated across or integrated into organelle membranes to avoid aggregation. Innovative cryo-EM methods are key to reveal the machineries that are responsible for processing different types of proteins and how they function at atomic detail. These approaches are a blueprint for detailed studies at native cellular membranes.
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