Instrumentation and Methodology Speakers

Philipp Pelz


Progress in atomic resolution 3D phase-contrast imaging
using 4D-STEM


Computational microscopy allows us to overcome limits imposed by imaging hardware. In this talk, I will present several milestones in overcoming the limits of electron microscopy of light-element contrast and depth of field at atomic resolution, allowing us to solve atomic structures in ever-increasing volumes with atomic resolution.

Kayla Nguyen


Breaking Resolution Limits with Ptychography using Topological Materials


Developments in direct electron detectors and algorithms for image reconstruction have formed a new generation of “computational lenses.”
 Using a computational lens approach, we can extract phase information and reconstruct images that go beyond the resolution of aberration-corrected electron microscopes. 
This work can be extended to uncovering new physics in topological condensed matter systems and provide accessible sub-angstrom resolution imaging capabilities for institutions that lack the funding or facility to host expensive, sensitive equipment. 

Jan Rusz


Simulations of phonon and magnon EELS/EEGS including dynamical effects and multiple inelastic excitations


Recent developments in STEM instrumentation enabled the STEM-EELS to explore new areas of physics, such as atomic vibrations at unprecedented spatial resolution.
Interpretation of these experiments calls for new theoretical and simulation methods. Resulting recent developments in simulations of STEM-EELS of phonons and magnons will be presented.

Berit Godge


Electron and x-ray spectroscopy for real-space electronic structure
mapping in superconducting nickelates 


Core-level spectroscopy is a powerful tool across myriad fields of materials research due to the access it provides to quantitative measurements of charge distribution, electronic structure, and bonding. Here I will highlight two advanced spectroscopies which add a further component of real-space mapping: STEM-EELS to localize at the atomic scale and sNIXS to directly resolve charge density distribution.

Lukas Palatinus


Exploiting dynamical diffraction theory in the crystal structure determination from 3D electron diffraction data


Taking the dynamical diffraction effects into account is a prerequisite for quantitatively fitting electron diffraction data. Incorporating these effects in the intensity calculation permits extracting fine details about crystal structures, including partial and mixed occupancies, charge density effects and absolute configuration of chiral species.

Peter Schweizer


Quantifying nanoscale diffusion phenomena using in situ TEM


Join us as we delve into the world of quantitative diffusion studies using chip-based in situ heating in TEM.
We will unravel the intricate dynamics of surface diffusion driven dewetting and follow the random walks of individual impurity atoms inside a crystal at atomic resolution.

Francesco Simone Ruggeri


Nano-chemical Imaging and Spectroscopy at the Single-molecule Level


The Ruggeri Lab develop and apply nanoscopic microscopy and spectroscopic technologies to study
biomolecular process in life and disease at the single molecule scale, as well as characterising advanced functional surfaces and materials. 

Chen Qian


Coupling Machine Learning with Electron Videography to Study Nanoscale Dynamics and Three-Dimensional Heterogeneity.


I will discuss our efforts in developing various machine-learning based imaging and data collection methods to facilitate the characterization of complex nanomaterials using liquid-phase TEM, electron tomography, and these two combined.

Martina Schifferer


Array tomography enables correlative volume electron microscopy and spatial transcriptomics”


Among all volume electron microscopy (EM) approaches, Array Tomography (AT) provides the unique advantage of tissue section restoration. This enables sample reinspection - an ideal feature for correlative and multimodal approaches. Here we present novel AT workflows that combine volume scanning EM with spatial transcriptomics and electron tomography and show their application in neurobiology.

Julia Mahamid


Enabling discovery by in-cell structural biology


Technological breakthroughs in cryo-electron tomography unlock an enormous potential for system-spanning discovery in structural cell biology

Adrian Wanner 


Near isotropic, high-resolution multi-beam scanning transmission electron microscopy with iterative milling


Multi-beam scanning transmission electron microscopy (mSTEM) of broad ion beam (BIB)
milled 250 nm thick sections is a fast and reliable vEM method suitable for the acquisition of mm³-sized samples.
It produces near isotropic, high-resolution stacks of each section by deconvolution of series of iteratively BIB-milled mSTEM images.

Yannick Schwab 


Precise targeting for volume electron microscopy, a multimodal approach. 


This talk will describe the targeting methods that are developed at EMBL. They rely on 3D maps built from fluorescence microscopy or X-ray imaging, and on specific workflows to accurately and semi-automatically approach the regions of interest prior to EM imaging. Example applications will show how to image selected regions of interest in multiple specimens including model and non-model organisms. 

Paul Guichard


Revealing the molecular architecture of the cell using Ultrastructure Expansion Microscopy (U-ExM)


Expansion microscopy is a recently developed technique that physically magnifies biological samples, enabling super-resolution visualisation of cells using a standard microscope. Here I will present the latest optimisations in this field, aimed at preserving cell ultrastructure in an optimal manner and revealing the cellular context previously invisible under fluorescence microscopy. To demonstrate the effectiveness of expansion microscopy, I will present various applications ranging from elucidating the architecture of the centriole to exploring its potential in gene therapy treatments.