Instrumentation and Methodology Speakers

Johan Verbeek


Unsupervised Quantification of large EELS datasets


EELS advanced considerably in past decades with more and higher quality data obtained by state of the art spectrometers. Extracting EELS information relies on user interaction which is time consuming and poses reproducibility risks. In this talk I demonstrate a fully automatic workflow resulting in fast and reliable results, without the sweat.

Philipp Pelz


Progress in atomic resolution 3D phase-contrast imaging
using 4D-STEM


Computational microscopy allows us to overcome limits imposed by imaging hardware. In this talk, I will present several milestones in overcoming the limits of electron microscopy of light-element contrast and depth of field at atomic resolution, allowing us to solve atomic structures in ever-increasing volumes with atomic resolution.

Kayla Nguyen


Breaking Resolution Limits with Ptychography using Topological Materials


Developments in direct electron detectors and algorithms for image reconstruction have formed a new generation of “computational lenses.”
 Using a computational lens approach, we can extract phase information and reconstruct images that go beyond the resolution of aberration-corrected electron microscopes. 
This work can be extended to uncovering new physics in topological condensed matter systems and provide accessible sub-angstrom resolution imaging capabilities for institutions that lack the funding or facility to host expensive, sensitive equipment. 

Penghan Lu 


Continuous-flow LHe-cooling TEM sample holder with high stability at sub-10K and in situ biasing functionality


A new continuous-flow LHe-cooling holder has been developed for in situ and operando cryogenic TEM applications. It is featured by very swift cool-down (1-2 min) and ultra-long cryo-hold time (>24 hours) at ultimately low temperature (5.2 K) with minimal thermal fluctuation (+/- 2.5 mK over hours) as well as in situ biasing and local heating functions with 6 (potentially more) electrical feedthrough.

Ondrej Krivanek 


Humphry Davy wrote, in 1812: “Nothing tends so much to the advancement of knowledge as the application of a new instrument.


We have developed several new instruments, which are contributing to the “advancement of knowledge”. They include a secondary electron (SE) STEM detector that has produced atomic resolution images of many different types of surfaces, and a High Energy Resolution Monochromated EELS System (HERMES), which is enabling vibrational studies of hitherto unobservable phenomena in the electron microscope.

Jan Rusz


Simulations of phonon and magnon EELS/EEGS including dynamical effects and multiple inelastic excitations


Recent developments in STEM instrumentation enabled the STEM-EELS to explore new areas of physics, such as atomic vibrations at unprecedented spatial resolution.
Interpretation of these experiments calls for new theoretical and simulation methods. Resulting recent developments in simulations of STEM-EELS of phonons and magnons will be presented.

Berit Goodge


Electron and x-ray spectroscopy for real-space electronic structure
mapping in superconducting nickelates 


Core-level spectroscopy is a powerful tool across myriad fields of materials research due to the access it provides to quantitative measurements of charge distribution, electronic structure, and bonding. Here I will highlight two advanced spectroscopies which add a further component of real-space mapping: STEM-EELS to localize at the atomic scale and sNIXS to directly resolve charge density distribution.

Lukas Palatinus


Exploiting dynamical diffraction theory in the crystal structure determination from 3D electron diffraction data


Taking the dynamical diffraction effects into account is a prerequisite for quantitatively fitting electron diffraction data. Incorporating these effects in the intensity calculation permits extracting fine details about crystal structures, including partial and mixed occupancies, charge density effects and absolute configuration of chiral species.

Colin Ophus 


Data analysis workflows to measure material properties and structure using 4D-STEM


Modern detectors can record millions of diffraction patterns in each
4D-STEM experiment. I will demonstrate how our py4DSTEM code can efficiently and robustly characterize metallic alloys, complex ferroelectric oxides,
2D heterostructures, soft matter samples, and other materials.

Peter Schweizer


Quantifying nanoscale diffusion phenomena using in situ TEM


Join us as we delve into the world of quantitative diffusion studies using chip-based in situ heating in TEM.
We will unravel the intricate dynamics of surface diffusion driven dewetting and follow the random walks of individual impurity atoms inside a crystal at atomic resolution.

Ido Kamine


From coherent amplification to quantum sensing in electron microscopy"


Recent breakthroughs involving quantum interactions of free electrons spawned an exciting new field: free-electron quantum optics.
We developed a platform for coherent free-electron interactions and demonstrated the first instance of coherent amplification in electron microscopy. Our experiments show a 20-fold contrast enhancement compared to conventional electron near-field imaging, resolving peak field amplitudes of few W/cm^2, in combined time-, space-, and phase-resolved measurements.
Our vision is to develop a microscope that can go beyond conventional imaging of matter to also image the quantum state of matter and probe quantum correlations between individual quantum systems.

Francesco Simone Ruggeri


Nano-chemical Imaging and Spectroscopy at the Single-molecule Level


The Ruggeri Lab develop and apply nanoscopic microscopy and spectroscopic technologies to study
biomolecular process in life and disease at the single molecule scale, as well as characterising advanced functional surfaces and materials. 

Chen Qian


Coupling Machine Learning with Electron Videography to Study Nanoscale Dynamics and Three-Dimensional Heterogeneity.


I will discuss our efforts in developing various machine-learning based imaging and data collection methods to facilitate the characterization of complex nanomaterials using liquid-phase TEM, electron tomography, and these two combined.

Arjen Jakobi 


Prospects of nanofluidic cavities for cryo-EM sample preparation


Micro-electromechanical systems (MEMS)-based nanofabrication technologies hold promise to automate the cryo-EM sample preparation
workflow and to unlock the potential of cryo-EM for 4D structural biology. 

Julia Mahamid


Enabling discovery by in-cell structural biology


Technological breakthroughs in cryo-electron tomography unlock an enormous potential for system-spanning discovery in structural cell biology

Martina Schifferer


Array tomography enables correlative volume electron microscopy and spatial transcriptomics”


Among all volume electron microscopy (EM) approaches, Array Tomography (AT) provides the unique advantage of tissue section restoration. This enables sample reinspection - an ideal feature for correlative and multimodal approaches. Here we present novel AT workflows that combine volume scanning EM with spatial transcriptomics and electron tomography and show their application in neurobiology.

Adrian Wanner 


Near isotropic, high-resolution multi-beam scanning transmission electron microscopy with iterative milling


Multi-beam scanning transmission electron microscopy (mSTEM) of broad ion beam (BIB)
milled 250 nm thick sections is a fast and reliable vEM method suitable for the acquisition of mm³-sized samples.
It produces near isotropic, high-resolution stacks of each section by deconvolution of series of iteratively BIB-milled mSTEM images.

Yannick Schwab 


Precise targeting for volume electron microscopy, a multimodal approach. 


This talk will describe the targeting methods that are developed at EMBL. They rely on 3D maps built from fluorescence microscopy or X-ray imaging, and on specific workflows to accurately and semi-automatically approach the regions of interest prior to EM imaging. Example applications will show how to image selected regions of interest in multiple specimens including model and non-model organisms. 

Ilaria Test


Dynamics of molecules at the nanoscale: from RESOLFT to STARSS


Fluorescence microscopy is pivotal to the investigation of the dynamics of macromolecules. We introduce new imaging methodologies based on photo-switching to address the dynamics of molecules at the nanoscale. 

Paul Guichard


Revealing the molecular architecture of the cell using Ultrastructure Expansion Microscopy (U-ExM)


Expansion microscopy is a recently developed technique that physically magnifies biological samples, enabling super-resolution visualisation of cells using a standard microscope. Here I will present the latest optimisations in this field, aimed at preserving cell ultrastructure in an optimal manner and revealing the cellular context previously invisible under fluorescence microscopy. To demonstrate the effectiveness of expansion microscopy, I will present various applications ranging from elucidating the architecture of the centriole to exploring its potential in gene therapy treatments.