LS-09 - CryoEM from membrane proteins to large complexes
1. Single-particle cryo-EM studies of membrane protein complexes: Membrane protein complexes are difficult samples to present optimally for single-particle cryo-EM studies with 3D reconstruction. Grids are typically prepared from detergent-solubilized samples or from samples based on lipid-based nanoparticles such as nanodiscs and saposin-lipid nanoparticles. Particular challenges are found in getting proper sampling of orientations, avoiding aggregation and denaturation at the air-water interface. The symposium will highlight recent developments in rational approaches to studies of challenging membrane protein complexes. 2. Molecular mechanisms by high resolution cryo electron microscopy: Recent advances in cryoEM allowed the deciphering of complex mechanisms performed by large protein complexes. Examples of this new trend can be seen in top journals every week.
Guillermo Montoya, University of Copenhagen
Poul Nissen, Aarhus University
Friedrich Förster, Universiteit Utrecht